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Pfizer TB Medium Base
with Glycerol, Egg Yolk, Glucose, and Malachite
Green

山東拓普生物工程有限公司 培養(yǎng)基配方 http://qp8008.cn

Composition per liter:

Agar ............................................................................................15.0g
Potato starch................................................................................15.0g
Casein acid hydrolysate..............................................................10.0g
K 2 HPO 4 .........................................................................................3.5g
Beef extract...................................................................................3.0g
L -Asparagine.................................................................................3.0g
Citric acid......................................................................................0.1g
Ferric ammonium citrate...............................................................0.1g
MgSO 4 ......................................................................................0.015g
Egg yolk emulsion.................................................................100.0mL
Glycerol ...................................................................................40.0mL
Malachite Green solution.........................................................13.0mL
Glucose solution........................................................................1.0mL
pH 7.0 ± 0.2 at 35°C
Source: This medium, without glycerol, glucose solution, Malachite
Green solution, and egg yolk emulsion, is available as a premixed pow-
der from HiMedia.
Egg Yolk Emulsion:
Composition per 100.0mL:
Chicken egg yolks..............................................................................9
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................25.0mL
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg. Beat to
form emulsion. Measure 50.0mL of egg yolk emulsion and add to
50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm
to 45°–50°C.
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................20.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Malachite Green Solution:
Composition per 20.0mL:
Malachite Green ...........................................................................0.2g
Preparation of Malachite Green Solution: Add Malachite
Green to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol and the other components,
except glucose solution, egg yolk emulsion, and Malachite Green solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 12 psi pressure–118°C. Cool to 55°C. Asepti-
cally add 100.0mL egg yolk emulsion, 1.0mL glucose solution, and
13.0mL Malachite Green solution. Mix thoroughly. Aseptically dis-
pense into sterile tubes. Allow to solidity as slants.
Use: For the cultivation of Mycobacterium tuberculosis.