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Pfizer Selective Enterococcus HiVeg Agar

山東拓普生物工程有限公司 培養(yǎng)基配方 http://qp8008.cn

Composition per liter:

Plant hydrolysate.........................................................................21.0g
Agar ............................................................................................15.0g
Plant peptone.................................................................................6.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Synthetic detergent........................................................................3.0g
Esculin ..........................................................................................1.0g
Sodium citrate...............................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
NaN 3 ...........................................................................................0.25g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation, cultivation, and enumeration of
Enterococcus species by the multiple tube technique.