- 定做培養(yǎng)基/定制培養(yǎng)基
- 顆粒培養(yǎng)基
- 標(biāo)準(zhǔn)菌株生化鑒定試劑盒
- 2020年版中國(guó)藥典
- 促銷(xiāo)/特價(jià)商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質(zhì))產(chǎn)品
- 按標(biāo)準(zhǔn)檢索培養(yǎng)基
- 預(yù)灌裝即用型成品培養(yǎng)基
- 模擬灌裝用培養(yǎng)基
- 干燥粉末培養(yǎng)基
- 培養(yǎng)基添加劑/補(bǔ)充劑
- 生化反應(yīng)鑒定管
- 染色液等配套產(chǎn)品
- 對(duì)照培養(yǎng)基/標(biāo)準(zhǔn)品
- 實(shí)驗(yàn)耗材與器具
- 生化試劑/化學(xué)試劑
- 菌種鑒定服務(wù)
DSMZ培養(yǎng)基383b組分A基礎(chǔ)/DSMZ383b組分A基礎(chǔ)
Desulfonauticus Medium Base,DSMZ medium 383b Solution A Base,DSMZ Medium 383b/DSMZ Medium383b/DSMZ 383b/DSMZ383b
  |
|
配套試劑
- 1. SD029 - 微量元素溶液SL-10/微量元素溶液SL10 Trace elements solution SL-10/Trace elements solution SL10
- 2. SD052 - 亞硒酸鹽-鎢酸鹽溶液 Selenite-tungstate solution
- 3. SD104 - 10%硫化鈉溶液（0.5g/支） 10% Na2S solution
- 4. SD092 - Wolin氏維生素溶液(10x) Wolin's vitamin solution(10x)
產(chǎn)品詳情
培養(yǎng)基配方（每升）
Na2SO4 3.0g
KH2PO4 0.2g
NH4Cl 0.3g
NaCl 21.0g
MgCl2·6H2O 3.0g
KCl 0.5g
CaCl2·2H2O 0.15g
Sodium resazurin(刃天青鈉)0.0005g
KH2PO4 0.2g
NH4Cl 0.3g
NaCl 21.0g
MgCl2·6H2O 3.0g
KCl 0.5g
CaCl2·2H2O 0.15g
Sodium resazurin(刃天青鈉)0.0005g
使用說(shuō)明
稱(chēng)取本品28.15g于970ml去離子水中，加入微量元素溶液SL-10(SD029)和亞硒酸鹽-鎢酸鹽溶液(SD052)各1支，攪拌溶解，通入80%H2和20%CO2的混合氣體不低于30分鐘使pH低于6.0，裝入?yún)捬跞萜髦校?21℃滅菌15分鐘。
制備DSMZ培養(yǎng)基383b時(shí)，另制備并加入溶液B(1g碳酸鈉、20ml水，121℃滅菌15分鐘)、C(1支SD092)以及D(取4mlSD104)，混合，使用無(wú)菌的酸堿溶液調(diào)節(jié)pH至7.0-7.2。備用
制備DSMZ培養(yǎng)基383b時(shí)，另制備并加入溶液B(1g碳酸鈉、20ml水，121℃滅菌15分鐘)、C(1支SD092)以及D(取4mlSD104)，混合，使用無(wú)菌的酸堿溶液調(diào)節(jié)pH至7.0-7.2。備用
特殊說(shuō)明
383b: DESULFONAUTICUS MEDIUM
1. Solution A is sparged with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at
least 30 min), then dispensed under the same gas atmosphere into anoxic Hungate-type
tubes or serum vials to 30% of their volume and autoclaved. Solution B is autoclaved
separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and E are autoclaved
under 100% N2 gas. Solution D is prepared under 100% N2 gas and filter-sterilized. To
complete the medium appropriate amounts of solutions B to E are added to the sterile
solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2.
2. After inoculation pressurize vials to 2 bar overpressure with sterile 80% H2 and 20% CO2
gas mixture.
3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution
freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the
beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.
For DSM 4206, DSM 15269: Supplement medium with 1.00 g/l Na-acetate, 2.00 g/l
Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from
sterile anoxic stock solutions prepared under N2 gas.
For DSM 15286, DSM 21156, DSM 101864: Supplement medium with 1.00 g/l Na-acetate
and 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium
from sterile and anoxic stock solutions. Vitamins are sterilized by filtration. Adjust pH of
medium to 6.5 - 6.7 prior to inoculation.
For DSM 111414: Adjust pH of final medium to 6.6.
Solution A
Solution B
Solution C
Wolin's vitamin solution (10x) 1.00 ml
Solution D
Selenite-tungstate solution (from medium 385)
Trace element solution SL-10 (from medium 320)
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally
make up to 1000.00 ml.
Wolin's vitamin solution (10x) (from medium 120)
p-Aminobenzoic acid 50.00 mg
(DL)-alpha-Lipoic acid 50.00 mg
Distilled water 1000.00 ml
|
Solution A Solution B Solution C Solution D |
972.00 20.00 1.00 13.00 |
ml ml ml ml |
least 30 min), then dispensed under the same gas atmosphere into anoxic Hungate-type
tubes or serum vials to 30% of their volume and autoclaved. Solution B is autoclaved
separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and E are autoclaved
under 100% N2 gas. Solution D is prepared under 100% N2 gas and filter-sterilized. To
complete the medium appropriate amounts of solutions B to E are added to the sterile
solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2.
2. After inoculation pressurize vials to 2 bar overpressure with sterile 80% H2 and 20% CO2
gas mixture.
3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution
freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the
beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.
For DSM 4206, DSM 15269: Supplement medium with 1.00 g/l Na-acetate, 2.00 g/l
Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from
sterile anoxic stock solutions prepared under N2 gas.
For DSM 15286, DSM 21156, DSM 101864: Supplement medium with 1.00 g/l Na-acetate
and 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium
from sterile and anoxic stock solutions. Vitamins are sterilized by filtration. Adjust pH of
medium to 6.5 - 6.7 prior to inoculation.
For DSM 111414: Adjust pH of final medium to 6.6.
Solution A
|
Na2SO4 KH2PO4 NH4Cl NaCl MgCl2 x 6 H2O KCl CaCl2 x 2 H2O Trace element solution SL-10 Selenite-tungstate solution Sodium resazurin (0.1% w/v) Distilled water |
3.00 0.20 0.30 21.00 3.00 0.50 0.15 1.00 1.00 0.50 970.00 |
g g g g g g g ml ml ml ml |
Solution B
|
Na2CO3 Distilled water |
1.00 20.00 |
g ml |
Wolin's vitamin solution (10x) 1.00 ml
Solution D
|
Na2S x 9 H2O Distilled water |
0.40 13.00 |
g ml |
|
NaOH Na2SeO3 x 5 H2O Na2WO4 x 2 H2O Distilled water |
0.50 3.00 4.00 1000.00 |
g mg mg ml |
|
HCl (25%) FeCl2 x 4 H2O ZnCl2 MnCl2 x 4 H2O H3BO3 CoCl2 x 6 H2O CuCl2 x 2 H2O NiCl2 x 6 H2O Na2MoO4 x 2 H2O Distilled water |
10.00 1.50 70.00 100.00 6.00 190.00 2.00 24.00 36.00 990.00 |
ml g mg mg mg mg mg mg mg ml |
make up to 1000.00 ml.
Wolin's vitamin solution (10x) (from medium 120)
|
Biotin Folic acid Pyridoxine hydrochloride Thiamine HCl Riboflavin Nicotinic acid Calcium D-(+)-pantothenate Vitamin B12 |
20.00 20.00 100.00 50.00 50.00 50.00 50.00 1.00 |
mg mg mg mg mg mg mg mg |
(DL)-alpha-Lipoic acid 50.00 mg
Distilled water 1000.00 ml
相關(guān)產(chǎn)品
- 1. MD468 - Thayer-Martin選擇性肉湯基礎(chǔ)改良Thayer-Martin選擇性肉湯基礎(chǔ) Thayer-Martin Selective Broth Base
- 2. MD429 - DSMZ培養(yǎng)基110a（不含葡萄糖、纖維二糖、麥芽糖和可溶性淀粉） DSMZ Medium 110a w/t Glucose，Cellobiose，Maltose&Starch
- 3. SD142 - 維生素溶液 Vitamin solution
- 4. MD239 - 無(wú)氮液體培養(yǎng)基 Nitrogen-Free Fluid Medium
- 5. MD225 - 克氏1號(hào)培養(yǎng)基
- 6. MD033 - 種子培養(yǎng)基
- 7. MD236 - SP4-Z培養(yǎng)基/ATCC培養(yǎng)基2764 ATCC Medium 2764/ATCC Medium2764/ATCC 2764/ATCC2764
- 8. MD286 - CDM培養(yǎng)基(金黃色葡萄球菌) Chemical Defined Medium for S.aureus
- 9. MD014P - 蛋黃培養(yǎng)基
- 10. SD151 - 0.1%刃天青鈉溶液 Sodium resazurin(0.1% w/v)
