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稱取本品1.443g于1L蒸餾水或去離子水中，補(bǔ)充1支SD038和2支SD039，攪拌溶解，分裝，121℃高壓滅菌15分鐘，冷至25℃，使用無(wú)菌10%碳酸氫鈉溶液調(diào)節(jié)pH至7.8，備用。

Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile
10% NaHCO3 (medium is turning from yellow to pink).
Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes
to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES
may be added to keep pH neutral for a longer time.
Aeration must be adjusted according to the density and activity of the culture. At low cell
concentrations (which is the case most of the time) cells are sensitive to strong aeration
(don’t shake the cultures).