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Toluidine Blue DNA Agar

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Toluidine Blue DNA Agar

山東拓普生物工程有限公司 培養(yǎng)基配方 http://qp8008.cn

Composition per liter:

Agar ............................................................................................ 10.0g
NaCl............................................................................................ 10.0g
Tris(hydroxymethyl)aminomethane buffer................................... 6.1g
Deoxyribonucleic acid (DNA)...................................................... 0.3g
Toluidine Blue O....................................................................... 0.083g
CaCl2, anhydrous....................................................................... 1.1mg
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except Toluidine Blue
O, to distilled/deionized water and bring volume to 1.0L. Mix thor
oughly. Gently heat and bring to boiling. Add Toluidine Blue O. Mix
thoroughly. Medium does not have to be sterilized if used immediately.
Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes
to cool in a slanted position.
Use: For the cultivation and differentiation of bacteria based on their
production of deoxyribonuclease (DNase). Bacteria that produce DNase
turn the medium pink.
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