[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2019-9-20] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidaminobacter Medium
Composition per liter:
NaHCO 3 ........................................................................................2.0g
Glycine..........................................................................................1.5g
NaCl..............................................................................................1.2g
KCl................................................................................................0.4g
MgCl 2 ·6H 2 O .................................................................................0.4g
Na 2 S·9H 2 O....................................................................................0.3g
KH 2 PO 4 .........................................................................................0.2g
Na 2 SO 4 ..........................................................................................0.2g
Yeast extract..................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.15g
Resazurin...................................................................................1.0mg
Na 2 SeO 3 ·5H 2 O..........................................................................30.0μg
Vitamin solution.......................................................................10.0mL
NaHCO 3 solution.......................................................................5.0mL
Na 2 S·9H 2 O solution...................................................................5.0mL
Trace elements solution SL-10..................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Lipoic acid .................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
NaHCO 3 Solution:
Composition per 5.0mL:
NaHCO 3 ........................................................................................0.5g
Preparation of NaHCO 3 Solution : Add NaHCO 3 to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Sparge un-
der 100% N 2 gas for 3 min. Autoclave for 15 min at 15 psi pressure–
121°C. Store under N 2 gas.
Na 2 S·9H 2 O Solution:
Composition per 5.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 5.0mL. Mix thoroughly.
Sparge under 100% N 2 gas for 3 min. Autoclave for 15 min at 15 psi
pressure–121°C. Store under N 2 gas.
Preparation of Medium: Add components, except vitamin solu-
tion, NaHCO 3  solution, and Na 2 S·9H 2 O solution, to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Boil for a few minutes. Allow to cool to room temperature
under 100% N 2 . Distribute into tubes or flasks under 100% N 2 . Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Before inoculation, aseptically and anaerobically add vitamin solution,
NaHCO 3  solution, and Na 2 S·9H 2 O solution. Mix thoroughly. Check
that final pH is 7.4.
Use: For the cultivation and maintenance of Acidaminobacter hydrog-
enoformans.