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Acid Glucose Salts Medium
山東拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Acid Glucose Salts Medium
Composition per liter:
Glucose.........................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................0.5g
(NH 4 ) 2 SO 4 ..................................................................................0.15g
KH 2 PO 4 .........................................................................................0.1g
KCl...........................................................................................50.0mg
Ca(NO 3 ) 2 ..................................................................................10.0mg
pH 3.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Thiobacillus organoparus.