[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-4-27] [發(fā)布人:李佳頤] [閱讀次數(shù):] [返回]

Lactobacillus Heteroferm Screen Broth
(MRS, Modified)
山東拓普生物工程有限公司 培養(yǎng)基配方 http://qp8008.cn
Glucose .......................................................................................20.0g
Proteose peptone No. 3...............................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
2-Phenylethyl alcohol...................................................................3.0g
Ammonium citrate........................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·H 2 O................................................................................0.05g
Bromcresol Green.......................................................................0.04g
Cycloheximide...........................................................................4.0mg
Tween™ 80................................................................................1.0mL
pH 4.3 ± 0.01 at 25°C
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.3 with
concentrated HCl. Distribute into test tubes that contain an inverted
Durham tube. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of Lactobacillus species from
foods.