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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Achromobacter Choline
Medium, Modified
Composition per liter:
NaCl............................................................................................30.0g
Agar............................................................................................15.0g
Choline chloride............................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
FeSO 4 ·7H 2 O..............................................................................0.018g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add agar, MgSO 4 ·7H 2 O, and
FeSO 4 ·7H 2 O to 500.0mL distilled/deionized water. Mix thoroughly.
Bring volume to 1.0L with distilled/deionized water. Gently heat and
bring to boiling. Add choline chloride. Mix thoroughly. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Achromobacter
cholinophagum.