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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acholeplasma Medium
(ATCC Medium 1215)
Composition per 1020.0mL:
PPLO broth without Crystal Violet........................................700.0mL
Fetal bovine serum, heat inactivated......................................100.0mL
Fresh yeast extract solution....................................................100.0mL
Tween™-glucose-BSA solution ............................................100.0mL
Phenol Red (0.1% solution).....................................................20.0mL
PPLO Broth without Crystal Violet:
Composition per 700.0mL:
Beef heart, infusion from..........................................................175.0g
Peptone..........................................................................................7.0g
NaCl..............................................................................................3.5g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet:  Add com-
ponents to distilled/deionized water and bring volume to 700.0mL. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Tween™-Glucose-BSA Solution:
Glucose .........................................................................................2.0g
Tween™ 80...................................................................................0.1g
Bovine serum albumin, fraction V (1% solution)..................100.0mL
Preparation of Tween™-Glucose-BSA Solution : Add glucose
and Tween™ 80 to 100.0mL of bovine serum albumin solution and mix
thoroughly. Filter sterilize solution through a 0.2μm membrane filter.
Preparation of Medium: Aseptically mix components. Distribute
into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Acholeplasma species.