[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-4-12] [發(fā)布人: ] [閱讀次數:] [返回]

HL Agar

培養(yǎng)基配方

Composition per plate:
Columbia agar base..................................................................10.0mL
Columbia blood top agar............................................................5.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Agar Base:
Composition per liter:
Agar ............................................................................................13.5g
Pancreatic digest of casein..........................................................12.0g
NaCl..............................................................................................5.0g
Peptic digest of animal tissue........................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Cornstarch.....................................................................................1.0g
Preparation of Columbia Agar Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Columbia Blood Top Agar:
Composition per liter:
Agar............................................................................................13.5g
Pancreatic digest of casein..........................................................12.0g
NaCl..............................................................................................5.0g
Peptic digest of animal tissue .......................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Cornstarch.....................................................................................1.0g
Horse blood, defibrinated ........................................................50.0mL
Preparation of Columbia Blood Top Agar: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse blood. Mix thoroughly.
Preparation of Medium:  Pour cooled, sterile Columbia agar base into sterile Petri dishes in 10.0mL volumes. Allow agar to solidify. Pour 5.0mL of cooled, sterile Columbia blood top agar over Columbia agar base that has solidified but is still warm.
Use: For the cultivation of Listeria monocytogenes.