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培養(yǎng)基配方
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HiFluoro™ Pseudomonas Agar Base

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-4-9] [發(fā)布人: ] [閱讀次數(shù):] [返回]

HiFluoro™ Pseudomonas Agar Base

培養(yǎng)基配方

Composition per liter:
Pancreatic digest of gelatin.........................................................18.0g
Agar ............................................................................................15.0g
K 2 SO 4 ..........................................................................................10.0g
Fluorogenic mixture....................................................................2.05g
MnCl 2 ............................................................................................1.4g
Cetrimide ......................................................................................0.3g
Glycerol ......................................................................................10.ml
pH 7.2 ± 0.2 at 25°C
Source: This medium, wihout glycerol, is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the selective isolation of Pseudomonas aeruginosa from clinical and nonclinical specimens by the fluorogenic method.
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