[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2020-7-13] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eugonic Agar

培養(yǎng)基配方
Composition per liter:
Agar.....................................................................15.0g
Casein enzymatic hydrolysate ............................................15.0g
Glucose...................................................................5.0g
Papaic digest of soybean meal.............................................5.0g
NaCl......................................................................4.0g
L -Cystine................................................................0.2g
Na2SO3 ...................................................................0.2g
Sheep blood, defibrinated...............................................50.0mL
pH 7.0 ± 2.0 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium:  Add components, except blood, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°C. Aseptically add 50.0mL of sterile defibri-
nated blood. Pour into sterile Petri dishes or leave in tubes.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation and maintenance of a variety of fastidious
microorganisms, e.g., Brucella, Haemophilus, Neisseria, Pasteurella,
and Lactobacillus species.