[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-13] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Euglena B 12 Medium

培養(yǎng)基配方
Composition per liter:
Sucrose...................................................................30.0g
L- Glutamic acid...........................................................6.0g
Glycine....................................................................5.0g
DL- Aspartic acid..........................................................4.0g
DL- Malic acid.............................................................2.0g
Succinic acid.............................................................1.04g
MgSO4 ·7H2O...............................................................0.8g
(NH4)2CO3 ................................................................0.72g
KH2PO4 ....................................................................0.6g
CaCO3 ....................................................................0.16g
FeCl3 ....................................................................0.06g
ZnSO4 ·7H2O..............................................................0.04g
Thiamine·HCl ...........................................................0.012g
MnSO4 ·H2O...............................................................6.0mg
CoSO4 ....................................................................5.0mg
(NH4)2MoO3 ..............................................................1.34mg
H3BO3 ...................................................................1.14mg
CuSO4 ·5H2O.............................................................0.62mg
pH 3.5 ± 0.1 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis-
solved. Distribute into tubes in 2.0mL volumes. Add standard solution
or test solution to each tube. Bring volume of each tube to 4.0mL with
distilled/deionized water. Autoclave for 15 min at 0 psi pressure–
100°C.
Use: For the assay of vitamin B 12 using Euglena gracilis as the test
organism.