[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-13] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eubacterium Medium

培養(yǎng)基配方
Composition per liter:
Beef brain powder.........................................................33.33g
Pancreatic digest of casein................................................15.0g
Yeast extract..............................................................10.0g
Glucose ....................................................................5.5g
Yeast extract...............................................................5.0g
NaCl........................................................................2.5g
Sodium thioglycolate........................................................1.8g
L- Cystine .................................................................0.5g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except beef brain pow-
der, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling under 97% N2 + 3% H2 .
Continue boiling for 15–20 min. Adjust pH to 7.0. Cool to 25°C under
97% N2 + 3% H2 . Anaerobically distribute into tubes in 9.0mL vol-
umes. Add 0.3g of beef brain powder to each tube. Cap tubes with rub-
ber stoppers. Place tubes in a press. Autoclave for 15 min at 15 psi
pressure–121°C with fast exhaust.
Use:  For the cultivation of Eubacterium species.