[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-9] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ethylene Production Agar for Mucor

培養(yǎng)基配方
Composition per liter:
Solution 3....................................................................700.0mL
Solution 1....................................................................100.0mL
Solution 2....................................................................100.0mL
Solution 4....................................................................100.0mL
Solution 1:
Composition per 100.0mL:
Agar, noble....................................................................10.0g
D -Glucose......................................................................5.0g
DL -Methionine .................................................................5.0g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Solution 2:
Composition per 100.0mL:
NaNO3 .......................................................................3.5g
Preparation of Solution 2: Add NaNO3 to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 3:
Composition per 700.0mL:
Citric acid.................................................................0.756g
MgSO4 ·7H2O..................................................................0.5g
NaOH........................................................................0.432g
CaCl2 ........................................................................0.1g
Ferric citrate·5H2O..........................................................0.1g
MnCl2 ·4H2O.................................................................0.05g
ZnSO4 ·7H2O.................................................................0.05g
CuCl2 ·2H2O ................................................................5.0mg
Na2MoO4 ·2H2O ..............................................................5.0mg
Na2B4O7 ·10H2O..............................................................2.0mg
CoCl2 ·6H2O ................................................................0.2mg
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 700.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 4:
Composition per 100.0mL:
KH2PO4 ......................................................................5.0g
Preparation of Solution 4: Add KH2PO4 to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Aseptically combine 100.0mL of sterile
solution 1,100.0mL of sterile solution 2,700.0mL of sterile solution 3,
and 100.0mL of sterile solution 4. Mix thoroughly. Pour into sterile Pe-
tri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of ethylene-producing
Mucor species.