[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-4-8] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Casman Agar Base with Rabbit Blood
(Casman-Medium)
(DSMZ Medium 439)

培養(yǎng)基配方
Composition per liter:
Noble agar..................................................14.0g
Proteose peptone No.3.......................................10.0g
Tryptose....................................................10.0g
NaCl.........................................................5.0g
Beef extract.................................................3.0g
Cornstarch...................................................1.0g
Glucose......................................................0.5g
p-Aminobenzoic acid.........................................0.05g
Nicotinamide................................................0.05g
Rabbit blood...............................................50.0mL
Water-lysed blood solution..................................1.5mL
pH 7.3 ± 0.2 at 25°C
Source: Casman agar base is available as a premixed powder from
BD Diagnostic Systems.
Water-Lysed Blood Solution:
Composition per 8.0mL:
Rabbit blood.................................................2.0mL
Preparation of Water-Lysed Blood Solution: Add blood to dis-
tilled/deionized water and bring volume to 8.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except rabbit blood
and water-lysed blood solution, to distilled/deionized water and bring
volume to 950.0L. Mix thoroughly. Gently heat to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add
50.0mL of sterile rabbit blood and 1.5mL of sterile water-lysed blood
solution. Water-lysed blood may be omitted if sterile blood is partially
lysed due to storage. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation and maintenance of Gardnerella vaginalis.