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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green 2%-Bile Broth, Fluorocult ®
(Fluorocult Brilliant Green 2%-Bile Broth)
(BRILA)

培養(yǎng)基配方
Composition per liter:
Ox bile, dried..............................................20.0g
Peptone ....................................................10.0g
Lactose.....................................................10.0g
L -Tryptophan ..............................................1.0g
4-Methylumbelliferyl-β- D -glucuronide.....................0.1g
Brilliant Green..........................................0.0133g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium:  Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Cool. Distribute into test tubes containing inverted Durham tubes.
Autoclave for 15 min at 15 psi pressure–121°C. Do not autoclave longer.
The prepared broth is clear and green.
Use : For the cultivation of Escherichia coli. Bile and Brilliant Green
almost completely inhibit the growth of undesired microbial flora, in
particular Gram-positive microorganisms. E. coli shows a positive flu-
orescence under UV light (366 nm). A positive indole reaction and, if
necessary, gas formation due to fermenting lactose, confirm the find-
ings.