展開

培養(yǎng)基配方

您現(xiàn)在的位置：網(wǎng)站首頁 >> 培養(yǎng)基配方

Yeast Synthetic Medium with 5′-dTMP

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-12-29] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://qp8008.cn

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Yeast nitrogen base without amino acids............6.7g

Casamino acids without vitamins .......................2.0g

2′-Deoxythymidine-5′-monophosphate ..........7.2mg

Preparation of Medium: Add glucose, yeast ni

trogen base without amino acids, casamino acids

without vitamins, and 2′-deoxythymidine-5′-mono

phosphate to distilled/deionized water and bring vol

ume to 200.0mL. Mix thoroughly. Filter sterilize.

Add agar to distilled/deionized water and bring vol

ume to 800.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°C. Aseptically combine the

two sterile solutions. Mix thoroughly. Pour into ster

ile Petri dishes or aseptically distribute into sterile

tubes.

Use: For the cultivation and maintenance of Saccharo

myces cerevisiae.

国产v欧美v亚洲v精品一区_日韩最新二区三区_午夜在线视频国产_国产无码免费看黄_麻豆成人av在线_精品三级电影视频高清_性色爽视频免费观看_东京热dvd无码一区二区三区_亚洲综合高清无码_最新毛片在线播放网站