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培養(yǎng)基配方
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Yeast Fermentation Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-12-29] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Yeast Fermentation Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://qp8008.cn
Composition per liter:
Peptone ...............................................................7.5g
Yeast extract........................................................4.5g
Bromthymol Blue (1.6% solution) ................. 1.0mL
Carbohydrate solution..................................... 1.0mL
Carbohydrate Solution:
Composition per 10.0mL:
Carbohydrate.......................................................0.6g
Preparation of Carbohydrate Solution: Add
carbohydrate to distilled/deionized water and bring
volume to 10.0mL. Glucose, maltose, lactose, galac
tose, or trehalose may be used. If raffinose is used, pre
pare a 12% solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept carbohydrate solution, to distilled/deionized wa
ter and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Distribute in 2.0mL vol
umes into test tubes that contain an inverted Durham
tube. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 1.0mL of sterile
carbohydrate solution. Mix thoroughly.
Use: For the cultivation and differentiation of yeast
based on carbohydrate fermentation patterns. Yeasts
that can ferment a specific carbohydrate turn the medi
um yellow.
