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Yeast Extract Peptone Starch Agar

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-12-29] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://qp8008.cn

Composition per liter:

Agar ..................................................................18.0g

Soluble starch....................................................10.0g

Peptone .............................................................10.0g

CaCO3 .................................................................5.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................3.0g

KH2PO4...............................................................0.5g

K2HPO4...............................................................0.5g

MgSO4·7H2O......................................................0.3g

Sodium citrate.................................................0.027g

NaCl..................................................................0.01g

MnSO4·5H2O....................................................0.01g

CuSO4·5H2O....................................................1.0mg

CoCl2·6H2O .....................................................1.0mg

FeSO4·7H2O.....................................................1.0mg

Preparation of Medium: Add components to tap

water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus

species that utilize starch as a carbon source.

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